We offer Kinase Substrates for direct, real-time(kinetic) Fluoroscent Microplate Assays that yield better, more reliable data than other methods, enabling better decisions regarding target biology and generation of lead candidates to empower faster movement through the drug discovery process.
Our Kinome Profiling & Compound Testing services come with the flexibility that can meet your HTS needs, including a variety of compound potency measurements and the option for preincubations, comparison of ATP at the Km or 1 mM, and analysis with recombinant enzyme or crude cell lysates. Our profiling services portfolio include Kinases,LIPID (DGK) Kinases & Phosphatases !
PhosphoSens® Technology forms the basis of its extensive portfolio of more than 400 unique Kinases and Phosphatase assays. We offer two assay formats: Continuous and End-Point/Red-Shift, compatible with 96, 384 and 1536-well plates and produce signals that are directly proportional to the amount of phosphorylated product present in the reaction mixture at the time of the measurement. In both cases, potency results have been shown to align with those obtained from cell-based assays, accelerating, and improving lead identification, characterization, profiling, and optimization studies, leading to the potential for developing drugs faster and with better efficacy.
HTS Kinase Profiling Assay Workflow with PhosphoSens Technology